7ZTW
Crystal Structure of Human Parechovirus 1 2A protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-06-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.548, 127.805, 54.282 |
Unit cell angles | 90.00, 95.19, 90.00 |
Refinement procedure
Resolution | 42.600 - 1.930 |
R-factor | 0.1867 |
Rwork | 0.184 |
R-free | 0.23840 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.411 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.600 | 1.980 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.057 | 0.635 |
Rpim | 0.053 | 0.579 |
Number of reflections | 42961 | 2808 |
<I/σ(I)> | 10.4 | 1.7 |
Completeness [%] | 99.3 | 98.6 |
Redundancy | 3.5 | |
CC(1/2) | 0.998 | 0.865 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1M Tris-HCl 0.2M Na acetate 10% glycerol 28% PEG1500 |