7ZTU
Crystal structure of the carotenoid-binding protein domain from silkworm Bombyx mori (BmCBP) in the apoform, D162L mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 63.175, 67.115, 121.123 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.000 - 1.900 |
| R-factor | 0.2084 |
| Rwork | 0.206 |
| R-free | 0.25140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ztq |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.158 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0349) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.000 | 46.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
| Rmerge | 0.179 | 0.234 | 1.573 |
| Rmeas | 0.203 | 0.272 | 1.776 |
| Rpim | 0.092 | 0.133 | 0.799 |
| Total number of observations | 88985 | 726 | 5751 |
| Number of reflections | 19947 | 173 | 1268 |
| <I/σ(I)> | 8.6 | 12.6 | 1.4 |
| Completeness [%] | 97.3 | 86.7 | 97.2 |
| Redundancy | 4.5 | 4.2 | 4.5 |
| CC(1/2) | 0.967 | 0.931 | 0.743 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.7 M Sodium citrate tribasic dihydrate, 0.1 M BIS- TRIS propane, pH 7.0 |
