7ZTR
Crystal structure of the carotenoid-binding protein domain from silkworm Bombyx mori (BmCBP) in the apoform, W232F mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-22 |
Detector | RIGAKU HyPix-6000HE |
Wavelength(s) | 1.54184 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 62.033, 66.772, 120.502 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.250 - 1.750 |
R-factor | 0.1836 |
Rwork | 0.181 |
R-free | 0.23230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ztq |
RMSD bond length | 0.015 |
RMSD bond angle | 2.092 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.7) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0349) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 60.250 | 60.250 | 1.780 |
High resolution limit [Å] | 1.750 | 9.090 | 1.750 |
Rmerge | 0.086 | 0.047 | 0.848 |
Rmeas | 0.090 | 0.052 | 0.952 |
Rpim | 0.027 | 0.021 | 0.418 |
Total number of observations | 289696 | 1212 | 5480 |
Number of reflections | 25252 | 216 | 1143 |
<I/σ(I)> | 20.8 | 39.5 | 1.8 |
Completeness [%] | 98.5 | 98.8 | 84.8 |
Redundancy | 11.5 | 5.6 | 4.8 |
CC(1/2) | 0.979 | 0.994 | 0.609 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0.5 M Sodium citrate tribasic dihydrate, 0.1 M BIS- TRIS propane, pH 7.0 |