7ZTQ
Crystal structure of the carotenoid-binding protein domain from silkworm Bombyx mori (BmCBP) in the apoform
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.873128 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 65.911, 67.326, 121.637 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.100 - 1.450 |
R-factor | 0.1458 |
Rwork | 0.143 |
R-free | 0.19310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5i9j |
RMSD bond length | 0.014 |
RMSD bond angle | 2.196 |
Data reduction software | DIALS |
Data scaling software | Aimless (0.7.4) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0349) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.100 | 47.100 | 1.480 |
High resolution limit [Å] | 1.450 | 7.950 | 1.450 |
Rmerge | 0.039 | 0.018 | 1.124 |
Rmeas | 0.043 | 0.020 | 1.267 |
Rpim | 0.017 | 0.009 | 0.569 |
Total number of observations | 300922 | 1737 | 10173 |
Number of reflections | 47986 | 340 | 2219 |
<I/σ(I)> | 20.7 | 70.5 | 1.3 |
Completeness [%] | 99.7 | 98.4 | 94.8 |
Redundancy | 6.3 | 5.1 | 4.6 |
CC(1/2) | 1.000 | 1.000 | 0.497 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 288 | 5% v/v Tacsimate, pH 7.0, 0.1 M HEPES, pH 7.0, 10% w/v Polyethylene glycol monomethyl ether 5000 |