7ZTB
Structure of the Salmonella tRNA pyrophosphokinase CapRel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97856 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.573, 136.196, 57.782 |
Unit cell angles | 90.00, 102.70, 90.00 |
Refinement procedure
Resolution | 68.100 - 2.312 |
R-factor | 0.2145 |
Rwork | 0.212 |
R-free | 0.26480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6s2t |
RMSD bond length | 0.008 |
RMSD bond angle | 0.970 |
Data reduction software | autoPROC |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.4 (3-FEB-2022)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.100 | 2.610 |
High resolution limit [Å] | 2.312 | 2.312 |
Rmerge | 0.115 | 1.292 |
Rmeas | 0.124 | |
Rpim | 0.046 | |
Number of reflections | 20877 | 1044 |
<I/σ(I)> | 11.2 | 1.5 |
Completeness [%] | 91.9 | |
Redundancy | 7.1 | |
CC(1/2) | 0.998 | 0.531 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277.16 | peg 20000, 20% |