7ZSW
F32V Cytochrome c prime beta from Methylococcus capsulatus (Bath): NO Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.90001 |
Spacegroup name | P 21 3 |
Unit cell lengths | 105.913, 105.913, 105.913 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.150 - 1.940 |
R-factor | 0.1885 |
Rwork | 0.187 |
R-free | 0.20660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6hih |
RMSD bond length | 0.013 |
RMSD bond angle | 2.211 |
Data reduction software | DIALS |
Data scaling software | xia2 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 61.150 | 61.170 | 1.980 |
High resolution limit [Å] | 1.940 | 5.280 | 1.950 |
Rmeas | 0.123 | 0.044 | 2.939 |
Rpim | 0.036 | 0.013 | 0.905 |
Total number of observations | 17536 | 15069 | |
Number of reflections | 29298 | 1570 | 1447 |
<I/σ(I)> | 13.5 | 61.2 | 0.5 |
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 11.4 | 11.2 | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 2 microlitres of 15 mg/ml protein in 0.1 M HEPES buffer, pH 7.5, mixed with with an equivalent volume of reservoir solution containing 0.01 M ZnSO4, 30% PEG 550 (v/v) and 0.1 M MES, pH 6.5. Cryoprotection in ML plus 10% glycerol |