7ZS9
Yeast RNA polymerase II transcription pre-initiation complex with the +1 nucleosome (complex A)
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.1 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |