7ZS6

Crystal structure of Apis mellifera RidA

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9724
Spacegroup name	I 41 2 2
Unit cell lengths	109.499, 109.499, 141.092
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.305 - 1.310
Rwork	0.131
R-free	0.16440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oni
RMSD bond length	0.017
RMSD bond angle	2.059
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MoRDa
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.305	1.330
High resolution limit [Å]	1.310	1.310
Rmerge	0.033	1.236
Number of reflections	101696	4890
<I/σ(I)>	14.8
Completeness [%]	99.4
Redundancy	4.4
CC(1/2)	0.999	0.404

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M Magnesium chloride hexahydrate, 0.1 M MES, 20% PEG 6000 pH 6