7ZS4
F32V Cytochrome c prime beta from Methylococcus capsulatus (Bath)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-12-01 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 3 |
Unit cell lengths | 105.674, 105.674, 105.674 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 74.710 - 1.750 |
R-factor | 0.1875 |
Rwork | 0.186 |
R-free | 0.21310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6hih |
RMSD bond length | 0.012 |
RMSD bond angle | 2.094 |
Data reduction software | DIALS |
Data scaling software | xia2 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 74.720 | 74.780 | 1.780 |
High resolution limit [Å] | 1.750 | 4.740 | 1.750 |
Rmeas | 0.073 | 0.022 | 2.284 |
Rpim | 0.028 | 0.008 | 0.868 |
Total number of observations | 14503 | 13618 | |
Number of reflections | 40057 | 2137 | 2016 |
<I/σ(I)> | 17.4 | 98.9 | 0.6 |
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 6.6 | 6.8 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 2 microlitres of 15 mg/ml protein in 0.1 M HEPES buffer, pH 7.5, mixed with with an equivalent volume of reservoir solution containing 0.01 M ZnSO4, 30% PEG 550 (v/v) and 0.1 M MES, pH 6.5. Cryoprotection in ML plus 10% glycerol |