7ZS2
Diheme cytochrome c Kustd1711 from Kuenenia stuttgartiensis, M292H mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.99988 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 62.650, 92.710, 55.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.510 - 1.120 |
R-factor | 0.1238 |
Rwork | 0.123 |
R-free | 0.13780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | WT Structure |
RMSD bond length | 0.019 |
RMSD bond angle | 1.526 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.150 |
High resolution limit [Å] | 1.120 | 1.120 |
Rpim | 0.042 | 0.892 |
Number of reflections | 211675 | 4298 |
<I/σ(I)> | 15.4 | 1.2 |
Completeness [%] | 88.6 | |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M calcium acetate, 0.1 M imidazole/HCl pH 8.0 and 20% PEG 1K |