7ZS1
Diheme cytochrome c Kustd1711 from Kuenenia stuttgartiensis, M292C mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-09-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.99988 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 61.210, 92.200, 55.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.570 - 1.700 |
R-factor | 0.1986 |
Rwork | 0.197 |
R-free | 0.22490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | WT Structure |
RMSD bond length | 0.008 |
RMSD bond angle | 0.951 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Rpim | 0.047 | 1.057 |
Number of reflections | 35153 | 2567 |
<I/σ(I)> | 14 | 1.5 |
Completeness [%] | 99.5 | |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M calcium acetate, 0.1 M imidazole/HCl pH 8.0 and 20% PEG 1K |