7ZS0
Diheme cytochrome c Kustd1711 from Kuenenia stuttgartiensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.71603 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 61.920, 92.290, 54.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.220 - 1.750 |
| R-factor | 0.1709 |
| Rwork | 0.169 |
| R-free | 0.19600 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.955 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rpim | 0.049 | 0.626 |
| Number of reflections | 58099 | 2755 |
| <I/σ(I)> | 20.3 | |
| Completeness [%] | 95.4 | |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M calcium acetate, 0.1 M imidazole/HCl pH 8.0 and 20% PEG 1K |
