7ZRA

Crystal structure of E.coli LexA in complex with nanobody NbSOS1(Nb14497)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-25
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0007
Spacegroup name	P 21 21 21
Unit cell lengths	59.918, 110.750, 231.796
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.970 - 2.800
Rwork	0.223
R-free	0.25870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jhc
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.020	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.050	0.250
Number of reflections	38956	3820
<I/σ(I)>	9.9	1.4
Completeness [%]	99.9	99.92
Redundancy	1.9
CC(1/2)	0.990	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.2 M calcium chloride, 0.1 M HEPES, 20 % w/v PEG 6000