7ZQY

Chaetomium thermophilum Rad50 Zn hook

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.2832
Spacegroup name	P 1
Unit cell lengths	43.490, 53.970, 110.420
Unit cell angles	75.85, 78.74, 90.00

Refinement procedure

Resolution	104.880 - 2.510
R-factor	0.2661
Rwork	0.264
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alphafold2
RMSD bond length	0.012
RMSD bond angle	1.779
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	104.880	2.540
High resolution limit [Å]	2.510	2.510
Rmerge	0.078
Number of reflections	63401	1715
<I/σ(I)>	7.86
Completeness [%]	97.4
Redundancy	3.55
CC(1/2)	0.930	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M MES, 0.6 M NaCl, 18% PEG4000