7ZQU
A fast recovering full-length LOV protein (DsLOV) from the marine phototrophic bacterium Dinoroseobacter shibae (Dark state) - C72A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-06 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.8726 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 91.454, 28.210, 48.018 |
Unit cell angles | 90.00, 119.40, 90.00 |
Refinement procedure
Resolution | 41.840 - 1.750 |
R-factor | 0.1886 |
Rwork | 0.188 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kuk |
RMSD bond length | 0.006 |
RMSD bond angle | 0.847 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19rc6_4061) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.840 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.125 | |
Rmeas | 0.136 | |
Rpim | 0.053 | 0.912 |
Number of reflections | 11015 | 593 |
<I/σ(I)> | 7.5 | 0.8 |
Completeness [%] | 99.8 | |
Redundancy | 6.4 | |
CC(1/2) | 0.996 | 0.462 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 0.1 M Tris, 0.2 M MgCl2, 20% PEG 8000 |