7ZQK

Crystal structure of photosynthetic glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii (CrGAPA) complexed with NAD+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-12
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00
Spacegroup name	C 2 2 2
Unit cell lengths	143.340, 147.100, 75.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.420 - 2.200
R-factor	0.25
Rwork	0.249
R-free	0.26590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rm4
RMSD bond length	0.008
RMSD bond angle	1.519
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	102.660	42.388	2.210
High resolution limit [Å]	2.100	6.640	2.100
Rmerge		0.153	0.559
Rmeas	0.237	0.171	0.633
Rpim	0.110	0.075	0.291
Total number of observations	186949	7718	27937
Number of reflections	46186	1608	6692
<I/σ(I)>	3.3	5.7	1.6
Completeness [%]	99.0	99.6	99.6
Redundancy	4	4.8	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.2-1.6 M (NH4)2SO4, 0.1 M Tris-HCl