7ZQ7
Structure of RpF-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.821, 148.805, 87.829 |
| Unit cell angles | 90.00, 112.11, 90.00 |
Refinement procedure
| Resolution | 81.370 - 3.000 |
| R-factor | 0.2199 |
| Rwork | 0.216 |
| R-free | 0.28870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m6n |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.370 | 3.047 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Number of reflections | 38043 | 1888 |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 99.5 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.970 | 0.630 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 12% 2-propanol, 100mM HEPES, 7.5 |
