7ZQ4
Crystal structure of photosynthetic glyceraldehyde-3-phosphate dehydrogenase from Chlamydomonas reinhardtii (CrGAPA) complexed with NADP+ and the oxidated catalytic cysteine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-12 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 142.396, 147.327, 74.758 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.180 - 1.700 |
R-factor | 0.2175 |
Rwork | 0.217 |
R-free | 0.22890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rm4 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.19) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.430 | 46.430 | 1.730 |
High resolution limit [Å] | 1.700 | 9.000 | 1.700 |
Rmerge | 0.083 | 0.050 | 1.197 |
Rmeas | 0.086 | 0.052 | 1.243 |
Rpim | 0.024 | 0.014 | 0.333 |
Total number of observations | 1108041 | 8188 | 60509 |
Number of reflections | 84958 | 663 | 4390 |
<I/σ(I)> | 17.5 | 43.4 | 1.8 |
Completeness [%] | 98.4 | 99.2 | 96.8 |
Redundancy | 13 | 12.3 | 13.8 |
CC(1/2) | 0.999 | 0.998 | 0.897 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 1.2-1.6 M (NH4)2SO4, 0.1 M Bicine pH 9.5 |