7ZPT

Crystal structure of MreB from Geobacillus stearothermophilus ATCC7953

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 1 21 1
Unit cell lengths	47.190, 62.010, 50.910
Unit cell angles	90.00, 112.98, 90.00

Refinement procedure

Resolution	46.870 - 1.800
R-factor	0.1931
Rwork	0.191
R-free	0.23380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4czj
RMSD bond length	0.008
RMSD bond angle	0.965
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.870	1.900
High resolution limit [Å]	1.800	1.800
Rmeas	0.150
Number of reflections	25002	3960
<I/σ(I)>	7.21
Completeness [%]	99.5
Redundancy	6.95
CC(1/2)	0.990	0.760

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	291	PEG 300, 2-(N-morpholino)ethanesulfonic acid