7ZPS
Cytochrome c prime beta from Methylococcus capsulatus (Bath): NO complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 105.660, 105.660, 105.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.250 - 1.560 |
| R-factor | 0.1817 |
| Rwork | 0.181 |
| R-free | 0.20240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hih |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.331 |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.5.23) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.250 | 47.250 | 1.600 |
| High resolution limit [Å] | 1.560 | 6.980 | 1.560 |
| Rmerge | 0.078 | 0.041 | 2.359 |
| Rmeas | 0.080 | 0.043 | 2.419 |
| Rpim | 0.018 | 0.010 | 0.533 |
| Total number of observations | 1109946 | 12971 | 84914 |
| Number of reflections | 56066 | 701 | 4133 |
| <I/σ(I)> | 20 | 64.6 | 1.5 |
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 19.8 | 18.5 | 20.5 |
| CC(1/2) | 0.999 | 0.998 | 0.546 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 2 microlitres of 15 mg/ml protein in 0.1 M HEPES buffer, pH 7.5, mixed with with an equivalent volume of reservoir solution containing 0.01 M ZnSO4, 35% PEG 550 (v/v) and 0.1 M MES, pH 6.5. Cryoprotection in ML plus 10% glycerol |
