7ZP8
70S E. coli ribosome with a stalled filamin domain 5 nascent chain
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | TFS KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.2 |
Software | RELION,CTFFIND |
Symmetry | C1 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |