7ZOU
Crystal structure of Synechocystis halorhodopsin (SyHR), Cl-pumping mode, ground state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.976 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 61.980, 61.980, 109.830 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.950 - 1.580 |
R-factor | 0.175 |
Rwork | 0.173 |
R-free | 0.20230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hyj |
RMSD bond length | 0.005 |
RMSD bond angle | 0.837 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.680 | 1.610 |
High resolution limit [Å] | 1.580 | 1.580 |
Rpim | 0.059 | 0.993 |
Number of reflections | 33368 | 1667 |
<I/σ(I)> | 8 | |
Completeness [%] | 96.9 | |
Redundancy | 10.6 | |
CC(1/2) | 0.998 | 0.225 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 4.6 | 293 | 1.6 M Ammonium Phosphate |