7ZOO
Carbohydrate binding domain CBM92-B from a multi-catalytic glucanase-chitinase from Chitinophaga pinensis DSM 2588 in complex with gentiobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX IV BEAMLINE BioMAX |
| Synchrotron site | MAX IV |
| Beamline | BioMAX |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.991873 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 44.621, 111.407, 70.536 |
| Unit cell angles | 90.00, 98.58, 90.00 |
Refinement procedure
| Resolution | 29.560 - 1.840 |
| R-factor | 0.1933 |
| Rwork | 0.191 |
| R-free | 0.21610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7zoh |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.920 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.560 | 1.910 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.060 | 0.400 |
| Number of reflections | 27998 | 2117 |
| <I/σ(I)> | 15.25 | 2.42 |
| Completeness [%] | 95.7 | 72.65 |
| Redundancy | 6.6 | |
| CC(1/2) | 0.999 | 0.947 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M citrate buffer pH 5.5 with 12.5 % w/v PEG 3350 |
