7ZNZ

Crystal structure of unliganded form of FucOB, a GH95 family alpha-1,2-fucosidase from Akkermansia muciniphila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	53.670, 100.400, 156.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.810 - 1.800
R-factor	0.1899
Rwork	0.189
R-free	0.21650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2eab
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.810	2.020
High resolution limit [Å]	1.800	1.950
Rmerge	0.050	0.550
Rmeas	0.052	0.574
Rpim	0.014	0.160
Number of reflections	152310	6192
<I/σ(I)>	32.63	4.82
Completeness [%]	100.0	99.98
Redundancy	13	12.8
CC(1/2)	1.000	0.959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	200 mM potassium fluoride, 20% (w/v) PEG 3500 (PEG ION HR2-126 protein crystallization screen, Hampton Research)