7ZNR
Inactive D62N mutant of BT1760 Endo-acting levanase from Bacteroides thetaiotaomicron VPI-5482
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-27 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97630 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 174.720, 174.720, 215.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 67.870 - 2.650 |
Rwork | 0.191 |
R-free | 0.24600 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.991 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | CRANK2 |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.870 | 2.740 |
High resolution limit [Å] | 2.650 | 2.650 |
Rpim | 0.911 | |
Number of reflections | 48504 | 4407 |
<I/σ(I)> | 12.1 | |
Completeness [%] | 99.9 | |
Redundancy | 16.3 | 15.2 |
CC(1/2) | 0.997 | 0.708 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 1.5 M lithium sulfate, 100 mM Hepes pH 7.5 |