7ZN1
Avidin + Biotin-Tempo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-10 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.353, 80.952, 60.951 |
| Unit cell angles | 90.00, 119.08, 90.00 |
Refinement procedure
| Resolution | 44.500 - 2.330 |
| R-factor | 0.2289 |
| Rwork | 0.226 |
| R-free | 0.27880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rav |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.500 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_4827) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.500 | 2.480 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Number of reflections | 40645 | 6570 |
| <I/σ(I)> | 5.13 | |
| Completeness [%] | 95.9 | |
| Redundancy | 2.13 | |
| CC(1/2) | 0.995 | 0.609 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298.15 | 0.2 M Zinc Acetate dihydrate 22% w/v PEG3350 |
