7ZN1
Avidin + Biotin-Tempo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-10 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 59.353, 80.952, 60.951 |
Unit cell angles | 90.00, 119.08, 90.00 |
Refinement procedure
Resolution | 44.500 - 2.330 |
R-factor | 0.2289 |
Rwork | 0.226 |
R-free | 0.27880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rav |
RMSD bond length | 0.003 |
RMSD bond angle | 0.500 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (dev_4827) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.500 | 2.480 |
High resolution limit [Å] | 2.330 | 2.330 |
Number of reflections | 40645 | 6570 |
<I/σ(I)> | 5.13 | |
Completeness [%] | 95.9 | |
Redundancy | 2.13 | |
CC(1/2) | 0.995 | 0.609 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298.15 | 0.2 M Zinc Acetate dihydrate 22% w/v PEG3350 |