7ZMX
Crystal structure of the Plant Homeodomain (PHD) of human ING3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 40.695, 40.695, 100.595 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.240 - 1.200 |
R-factor | 0.1743 |
Rwork | 0.172 |
R-free | 0.20440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vnf |
RMSD bond length | 0.009 |
RMSD bond angle | 0.999 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.240 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.085 | 0.613 |
Rmeas | 0.094 | 0.723 |
Rpim | 0.032 | 0.414 |
Number of reflections | 47743 | 51 |
<I/σ(I)> | 18.59 | 0.18 |
Completeness [%] | 92.4 | 45.6 |
Redundancy | 14.8 | 5.5 |
CC(1/2) | 0.990 | 0.760 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 295 | Protein solution: ING3 PHD at 20 mg/ml in Tris 20 mM pH = 6.5, 150 mM NaCl, 1 mM DTT. Reservoir solution: 0.1 M Tris pH = 8.4, 30% PEG 4000, 0.2 M calcium chloride Mixture of 1 ul of protein solution with 1 ul of reservoir solution. |