7ZMU
14-3-3s binding to non-natural peptide 2d
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-05-04 |
Detector | DECTRIS EIGER2 S 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 82.278, 112.007, 62.680 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.550 - 1.600 |
R-factor | 0.2557 |
Rwork | 0.254 |
R-free | 0.29370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jc3 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.550 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 73822 | 1880 |
<I/σ(I)> | 8.2 | 3.5 |
Completeness [%] | 100.0 | |
Redundancy | 13.4 | |
CC(1/2) | 0.988 | 0.917 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 277 | 95 mM HEPES pH 7.1, 0.19 M CaCl2, 5% glycerol and 27% PEG400 |