7ZM6
Nariva virus receptor binding protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.382, 82.957, 92.988 |
| Unit cell angles | 90.00, 90.86, 90.00 |
Refinement procedure
| Resolution | 61.928 - 2.070 |
| R-factor | 0.2317 |
| Rwork | 0.230 |
| R-free | 0.26970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Mossman virus receptor binding protein |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_3488) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.960 | 2.140 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.180 | 0.810 |
| Rpim | 0.130 | 0.590 |
| Number of reflections | 51334 | 50856 |
| <I/σ(I)> | 4.2 | 1.3 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 3.3 | |
| CC(1/2) | 0.980 | 0.620 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M magnesium chloride, 0.1 M HEPES pH 7.5, 25% PEG 3350, 10% PEG 400 |
