7ZLW
NME1 in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-20 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.966000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.656, 70.193, 113.167 |
| Unit cell angles | 90.00, 90.96, 90.00 |
Refinement procedure
| Resolution | 59.650 - 2.200 |
| R-factor | 0.1738 |
| Rwork | 0.171 |
| R-free | 0.22010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hvd |
| Data reduction software | XDS (Jan 26, 2018) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.650 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.028 | 0.309 |
| Rmeas | 0.039 | 0.437 |
| Rpim | 0.028 | 0.309 |
| Number of reflections | 40517 | 3536 |
| <I/σ(I)> | 11.4 | 1.7 |
| Completeness [%] | 98.2 | 98.9 |
| Redundancy | 2.5 | 2.5 |
| CC(1/2) | 0.999 | 0.877 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | PEG 400, NaCl, Tris |
