7ZKW
Crystal structure of cystinosin from Arabidopsis thaliana in complex with Cystine and sybody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 283.372, 64.065, 55.443 |
| Unit cell angles | 90.00, 99.95, 90.00 |
Refinement procedure
| Resolution | 69.778 - 3.372 |
| Rwork | 0.249 |
| R-free | 0.27950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7zk1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.006 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0350) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.780 | 3.520 |
| High resolution limit [Å] | 3.370 | 3.370 |
| Rpim | 0.150 | |
| Number of reflections | 13144 | 3643 |
| <I/σ(I)> | 2 | 0.6 |
| Completeness [%] | 100.0 | |
| Redundancy | 6.1 | |
| CC(1/2) | 0.790 | 0.100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5.5 | 293 | 28% PEG 500DME, 100 mM MES-NaOH, pH 5.50, 150 mM K-formate |
