7ZKT
Moss spermine/spermidine acetyl transferase (PpSSAT) in complex with CoA and lysine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.010, 138.430, 129.780 |
| Unit cell angles | 90.00, 94.03, 90.00 |
Refinement procedure
| Resolution | 94.550 - 2.060 |
| R-factor | 0.177 |
| Rwork | 0.176 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7zhc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.550 | 2.291 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.161 | 1.200 |
| Number of reflections | 138210 | 4684 |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 98.2 | |
| Redundancy | 6.2 | |
| CC(1/2) | 0.996 | 0.579 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.2 M ammonium sulfate, 0.1 M trisodium citrate pH 5.6, 15 % PEG 4000 |
