7ZJV

Structure of human USPL1 in covalent complex with DeltaN-SUMO2/3-PA probe

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-22
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0000
Spacegroup name	P 41 21 2
Unit cell lengths	95.708, 95.708, 82.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.800 - 2.400
R-factor	0.2316
Rwork	0.230
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7zju
RMSD bond length	0.005
RMSD bond angle	1.133
Data reduction software	xia2 (0.5.902)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.800	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.053	0.858
Rmeas	0.057	0.933
Number of reflections	28746	1597
<I/σ(I)>	18.2	1.8
Completeness [%]	100.0
Redundancy	9.1
CC(1/2)	0.999	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.9	293	100 mM CHES pH 8.9, 34% PEG600