7ZJ1
Crystal structure of ADAR1-dsRBD3 dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-12 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 44.235, 44.235, 131.803 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.150 - 1.650 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.22470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2mdr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.081 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | PHENIX (1.19.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.150 | 19.150 | 1.709 |
| High resolution limit [Å] | 1.650 | 9.040 | 1.650 |
| Rmerge | 0.152 | 0.083 | 0.867 |
| Rmeas | 0.156 | 0.087 | 0.900 |
| Rpim | 0.035 | 0.022 | 0.238 |
| Number of reflections | 18792 | 138 | 1852 |
| <I/σ(I)> | 12.45 | 23 | |
| Completeness [%] | 99.3 | 90.8 | 99.84 |
| Redundancy | 18.7 | 15.1 | 14.2 |
| CC(1/2) | 0.995 | 0.994 | 0.585 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 100 mM sodium citrate pH 4.0, 20% (w/v) PEG 6000, and 1.0 M LiCl |
