7ZIT

14-3-3 in complex with SARS-COV2 N phospho-peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-28
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.541870
Spacegroup name	P 21 21 21
Unit cell lengths	72.180, 83.517, 111.356
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.320 - 1.790
R-factor	0.1849
Rwork	0.184
R-free	0.20510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nas
RMSD bond length	0.007
RMSD bond angle	0.887
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.320	1.854
High resolution limit [Å]	1.790	1.790
Rmerge	0.030	0.412
Number of reflections	62474	5637
<I/σ(I)>	21.6
Completeness [%]	97.4
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	9 0.2 M Sodium acetate 0.1 M Sodium cacodylate pH 6.5 30% (w/v) PEG 8000