7ZIN
JC Polyomavirus VP1 in complex with 6'-Sialyllactose glycomacromolecules (aliphatic linker)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2021-12-12 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.99 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.132, 96.692, 128.449 |
| Unit cell angles | 90.00, 110.63, 90.00 |
Refinement procedure
| Resolution | 44.855 - 1.648 |
| Rwork | 0.166 |
| R-free | 0.19140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nxd |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.535 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.750 |
| High resolution limit [Å] | 1.648 | 1.650 |
| Rmeas | 0.150 | 1.410 |
| Number of reflections | 204346 | 32369 |
| <I/σ(I)> | 10.69 | 1.31 |
| Completeness [%] | 98.8 | |
| Redundancy | 6.85 | |
| CC(1/2) | 0.990 | 0.590 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES pH 7.5, 0.2 M KSCN, 12% (w/v) PEG 3350 |
