7ZIE
Gcf1p, multimerizes and bridges the mitochondrial DNA from Candida albicans by a specific mechanism.
Refinement Statistics
Experimental method: SOLUTION SCATTERING, X-RAY DIFFRACTION (2.9 Å)
| Cell axes | 66.630 | 96.900 | 113.060 |
| Cell angles | 90.00 | 90.00 | 90.00 |
| Spacegroup | P 2 21 21 | ||
| Resolution limits | 73.57 - 2.90 | ||
Crystallization Conditions
| crystal ID | method | pH | pH range | temperature | unit |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 |
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.981664, 1.0 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 66.630, 96.900, 113.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.570 - 2.900 |
| R-factor | 0.2164 |
| Rwork | 0.211 |
| R-free | 0.26310 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.988 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.570 | 3.004 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.037 | |
| Number of reflections | 16672 | 1587 |
| <I/σ(I)> | 10.07 | 0.76 |
| Completeness [%] | 98.6 | 92.55 |
| Redundancy | 1.9 | 1.9 |
| CC(1/2) | 1.000 | 0.319 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 20% PEG 1000 0.1M Na/K-phosphate pH 6.2 0.1M NaCl 3% Glycerol |
