7ZHX
Leishmania donovani Glucose 6-Phosphate Dehydrogenase (N-terminal deletion variant)complexed with NADP(H)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 75.750, 229.660, 83.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.300 - 1.900 |
R-factor | 0.1843 |
Rwork | 0.182 |
R-free | 0.21030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | D_1292122100 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.982 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.300 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 58616 | 32174 |
<I/σ(I)> | 18.9 | |
Completeness [%] | 99.7 | |
Redundancy | 5.6 | |
CC(1/2) | 1.000 | 0.800 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 283 | 6 % PEG 3000, 200 mM AmCl2 |