7ZHW
Leishmania donovani Glucose 6-Phosphate Dehydrogenase complexed with NADP(H) and Glucose 6-Phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9999 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 215.830, 66.770, 121.020 |
Unit cell angles | 90.00, 120.71, 90.00 |
Refinement procedure
Resolution | 49.300 - 3.300 |
R-factor | 0.2386 |
Rwork | 0.236 |
R-free | 0.26250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | D_1292122100 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.615 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.300 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 22716 | 11462 |
<I/σ(I)> | 9.7 | 1.3 |
Completeness [%] | 99.1 | 98.6 |
Redundancy | 5.1 | |
CC(1/2) | 1.000 | 0.700 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 283 | 12 % PEG 3000 and 200 mM ammonium sulfate |