7ZHV
Leishmania donovani Glucose 6-Phosphate Dehydrogenase complexed with Glucose 6-Phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 218.740, 67.820, 120.750 |
| Unit cell angles | 90.00, 121.67, 90.00 |
Refinement procedure
| Resolution | 39.100 - 3.300 |
| R-factor | 0.2562 |
| Rwork | 0.250 |
| R-free | 0.30900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | D_1292122100 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.187 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.100 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 22384 | 2211 |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 96.7 | |
| Redundancy | 2.9 | |
| CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | 12 % PEG 3000 and 200 mM ammonium sulfate |
