7ZHT
Leishmania donovani Glucose 6-Phosphate Dehydrogenase apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9998 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 117.690, 65.820, 189.220 |
Unit cell angles | 90.00, 92.35, 90.00 |
Refinement procedure
Resolution | 39.200 - 2.800 |
R-factor | 0.2499 |
Rwork | 0.244 |
R-free | 0.30400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ukw |
RMSD bond length | 0.009 |
RMSD bond angle | 1.711 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.200 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.100 | |
Number of reflections | 70723 | 70723 |
<I/σ(I)> | 8.6 | 0.7 |
Completeness [%] | 98.1 | |
Redundancy | 2.8 | |
CC(1/2) | 1.000 | 0.500 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 283 | 18 % PEG 3000, 200 mM ammonium chloride. |