7ZHT
Leishmania donovani Glucose 6-Phosphate Dehydrogenase apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9998 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 117.690, 65.820, 189.220 |
| Unit cell angles | 90.00, 92.35, 90.00 |
Refinement procedure
| Resolution | 39.200 - 2.800 |
| R-factor | 0.2499 |
| Rwork | 0.244 |
| R-free | 0.30400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ukw |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.711 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.200 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.100 | |
| Number of reflections | 70723 | 70723 |
| <I/σ(I)> | 8.6 | 0.7 |
| Completeness [%] | 98.1 | |
| Redundancy | 2.8 | |
| CC(1/2) | 1.000 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | 18 % PEG 3000, 200 mM ammonium chloride. |
