7ZHS
3D reconstruction of the cylindrical assembly of DnaJA2 delta G/F by imposing D5 symmetry
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 6.9 |
| Number of Particle | 288620 |
| Software | crYOLO,RELION,REFMAC,EPU,Gctf |
| Symmetry | D5 |
| CTF correction | NONE |
