7ZHH

Complex structure of drosophila Unr CSD789 and a poly(A) RNA sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.976
Spacegroup name	C 1 2 1
Unit cell lengths	97.920, 36.780, 85.830
Unit cell angles	90.00, 119.81, 90.00

Refinement procedure

Resolution	48.790 - 1.600
R-factor	0.1884
Rwork	0.187
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6y4h
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.790	48.790	1.640
High resolution limit [Å]	1.600	7.150	1.600
Rmerge	0.089	0.030	1.257
Rmeas	0.096	0.032	1.364
Total number of observations	235709
Number of reflections	35311	434	2517
<I/σ(I)>	12.94	45.46	1.35
Completeness [%]	99.5	97.7	97.2
Redundancy	6.675	6.203	6.631
CC(1/2)	0.999	0.999	0.492

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M Tris-Cl (pH 7.0) 0.2 M lithium sulfate 2 M ammonium sulfate