7ZHG
High-resolution cryo-EM structure of Pyrococcus abyssi 30S ribosomal subunit bound to mRNA and initiator tRNA anticodon stem-loop
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | TFS KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 2.25 |
Number of Particle | 2000000 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |