7ZGV

Serratia NucC bound to cA3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-24
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	114.265, 114.265, 96.069
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.850 - 1.480
R-factor	0.159
Rwork	0.158
R-free	0.18110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6q1h
RMSD bond length	0.011
RMSD bond angle	1.133
Data reduction software	XDS (BUILT=20190806)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.210	1.506
High resolution limit [Å]	1.480	1.480
Rmerge	0.117	1.517
Rpim	0.027	0.354
Number of reflections	40117	1987
<I/σ(I)>	17.6
Completeness [%]	100.0
Redundancy	19.9
CC(1/2)	0.999	0.773

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Tris pH 7.5 0.2 M calcium acetate 25% PEG MME 2000