7ZGN
Plant/insect N-glycan active PNGase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.050, 100.550, 180.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.379 - 1.950 |
Rwork | 0.189 |
R-free | 0.23950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4r4z |
RMSD bond length | 0.011 |
RMSD bond angle | 1.768 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0326) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.090 | 1.990 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 79080 | 4454 |
<I/σ(I)> | 12.6 | 1.7 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 7.2 | 7.2 |
CC(1/2) | 0.999 | 0.517 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10 mM Cobalt chloride hexahydrate, 100 mM Tris pH 8.5 and 10% w/v Polyvinylpyrrolidone K15 |