7ZGI

chloroplast trigger factor (TIG1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97779
Spacegroup name	C 1 2 1
Unit cell lengths	175.291, 69.879, 106.043
Unit cell angles	90.00, 110.99, 90.00

Refinement procedure

Resolution	54.310 - 2.600
R-factor	0.2371
Rwork	0.235
R-free	0.28260
Structure solution method	SAD
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	SHELXCD
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.310	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.094	0.548
Number of reflections	37064	4515
<I/σ(I)>	10.2
Completeness [%]	99.8
Redundancy	6.6
CC(1/2)	0.996	0.941

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.3 M MgSO4, 10 % PEG 3350