7ZGI
chloroplast trigger factor (TIG1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-29 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97779 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 175.291, 69.879, 106.043 |
Unit cell angles | 90.00, 110.99, 90.00 |
Refinement procedure
Resolution | 54.310 - 2.600 |
R-factor | 0.2371 |
Rwork | 0.235 |
R-free | 0.28260 |
Structure solution method | SAD |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | SHELXCD |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.310 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.094 | 0.548 |
Number of reflections | 37064 | 4515 |
<I/σ(I)> | 10.2 | |
Completeness [%] | 99.8 | |
Redundancy | 6.6 | |
CC(1/2) | 0.996 | 0.941 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.3 M MgSO4, 10 % PEG 3350 |