7ZGE
BrxA, BREX phage defence protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 174.424, 42.539, 86.839 |
| Unit cell angles | 90.00, 102.74, 90.00 |
Refinement procedure
| Resolution | 42.530 - 2.090 |
| R-factor | 0.2249 |
| Rwork | 0.223 |
| R-free | 0.26510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bhw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.116 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.530 | 2.150 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.079 | 0.374 |
| Number of reflections | 37354 | 2867 |
| <I/σ(I)> | 4.4 | |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.2 M (NH4)2SO4, 0.1 M N-(2-acetamido)iminodiacetic acid (ADA, pH 6.5), 18% v/v PEG Smear High |
