7ZGD

Structure of yeast Sec14p with NPPM244

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	124
Detector technology	PIXEL
Collection date	2018-04-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	86.728, 86.728, 109.610
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.272 - 2.080
R-factor	0.1992
Rwork	0.198
R-free	0.23160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aua
RMSD bond length	0.008
RMSD bond angle	0.895
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.272	6.190
High resolution limit [Å]	2.080	2.080
Rmeas	0.109	0.104
Number of reflections	55450	55450
<I/σ(I)>	14.52
Completeness [%]	100.0
Redundancy	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		294	129,5 mM sodium acetate, 64,8 mM TRIS, 4,6 % (w/v) PEG 4000, and 11.9 % (v/v) glycerol adjusted to pH 7.0