7ZG9
Structure of yeast Sec14p with himbacine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 238 |
| Detector technology | PIXEL |
| Collection date | 2018-04-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 86.593, 86.593, 148.746 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.297 - 1.764 |
| R-factor | 0.1963 |
| Rwork | 0.195 |
| R-free | 0.22160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aua |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.088 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.297 | 5.260 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmeas | 0.072 | 0.072 |
| Number of reflections | 122833 | 122833 |
| <I/σ(I)> | 19.16 | |
| Completeness [%] | 100.0 | |
| Redundancy | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 294 | 129,5 mM sodium acetate, 64,8 mM TRIS, 4,6 % (w/v) PEG 4000, and 11.9 % (v/v) glycerol, pH 7.0 |
